Journal: Journal of Cerebral Blood Flow & Metabolism
Article Title: Concentration, distribution, and influence of aging on the 18 kDa translocator protein in human brain: Implications for brain imaging studies
doi: 10.1177/0271678X19858003
Figure Lengend Snippet: Quantification of TSPO protein in autopsied human brain. (a) Immunoblots of TSPO in the pooled tissue standards, in the commercial N-His-tagged recombinant human TSPO-3462H and in human adrenal samples with monoclonal (EPR5384, 1:100K and MA5-24844, 1:30K dilution) and polyclonal (PA5-75544, 1:10K) antibodies. (b) Standard curves for the recombinant TSPO. Note more abundant TSPO in adrenal than in brain for both EPR5384 and MA5-24844, polymer protein bands of the recombinant TSPO, high molecular weight non-specific reactions for PA5-75544, and detection of a TSPO fragment but not 18 kDa TSPO by PA5-75544 in human adrenal samples.
Article Snippet: Abnova H00000706-M01 Mouse IgG 1k (3D8-B2) Unknown NA No major band identified Santa Cruz Biotech Sc-20120 rPolyAb (FL-169) Unknown NA No major band identified GFAP (50,48,45,and 43 kDa) Chemicon/Millipore MAB360 Mouse IgG 1 Unknown 1:100K Vimentin (57 kDa) Santa Cruz Biotech Sc-6260 Mouse IgG 1 Unknown 1:2K 2 nd antibodies Anti-rabbit IgG-HRP Southern Biotech 4050-05 Goat IgG 1:10-20K Anti-mouse IgG1-HRP Southern Biotech 1070-05 Goat IgG 1:10K Anti-goat IgG-HRP Southern Biotech 6160-05 Rabbit IgG 1:10K Recombinant TSPO Human TSPO, N-His Creative BioMart TSPO-3462H / / 0.1 ng 21 kDa Human TSPO, N-His/GST Creative BioMart TSPO-1696H / / <1 ng 48.8 kDa (crude lysate) Human TSPO, C-Myc/DDK Origene TP320107 / / 40 ng 23 kDa Mouse TSPO, N-His Jean J. Lacapere Gift / / 1 ng 21 kDa Mouse TSPO, C-Myc/DDK Origene TP605624 / / NA 23 kDa Rat TSPO, C-Myc/DDK Origene TP605625 / / NA 23 kDa Open in a separate window Antibody and recombinant protein sources and conditions used.
Techniques: Western Blot, Recombinant, Molecular Weight